eCM (Eur Cell Mater / e Cells & Materials) Not-for-profit Open Access
Created by Scientists, for Scientists
 ISSN:1473-2262         NLM:100973416 (link)         DOI:10.22203/eCM

2001   Volume No 2 - pages 1-9

Title: The influence of pore geometry in cp Ti-implants a cell culture investigation

Authors: R. Stangl, B. Rinne, S. Kastl and C. Hendrich

Address: Department of Traumatology, Surgical Clinic, University of Erlangen; Department of Experimental Dental Medicine, University of Wrzburg, and Department of Orthopaedics, University of Wrzburg, Germany.

E-mail: richard.stangl at

Key Words: Topography, copper vapor laser, porous geometry, cell culture, titanium, osteoblasts.

Publication date: 12th July 2001

Abstract: Biocompatibility testing of differently structured titanium implants was performed using an in vitro test system of a newly established human fetal osteoblastic cell line (hFOB 1.19). Cell adhesion of osteoblastic cells on the different porous geometries and the suitability of a copper vapor laser system for surface structuring was tested with the following parameters: cell-number, cell viability, alkaline phosphatase expression.
The analysis of the cell culture results demonstrated that 25m and 200m porous geometries showed similar or even better results than the negative control of polystyrene; there was no sign of toxic effects. However, the 100m porous geometry showed an impressive negative influence on the calculated parameters. The reason for this effect is unclear.
The series with 50m, 300m, 400m and 500m showed a comparable, intermediate effect on the cell culture with respect to the different parameters. However, the results were worse than with the 25 and 200m porous geometry. In conclusion, the 25m and 200m porous geometry seems to have the most positive effect on the human osteoblastic cell line hFOB 1.19.

Article download: Pages 1-9 (PDF file)
DOI: 10.22203/eCM.v002a01