eCM (Eur Cell Mater / e Cells & Materials) Not-for-profit Open Access
Created by Scientists, for Scientists
 ISSN:1473-2262         NLM:100973416 (link)         DOI:10.22203/eCM

2004   Volume No 7- pages 42-51

Title: Comparison of Microfocus- and Synchrotron X-ray Tomography for the Analysis of Osteointegration around Ti6Al4V Implants 

Authors: R. Bernhardt, D. Scharnweber, B. Müller, P. Thurner, H. Schliephake, P. Wyss, F. Beckmann, J. Goebbels, and H. Worch

Address: B. Müller, Computer Vision Laboratory, Swiss Federal Institute of Technology, CH-8092 Zürich, Switzerland

E-mail: bmueller at

Key Words: X-ray micro computed tomography, comparison of microfocus and synchrotron radiation, osteointegration, surface modification of titanium implants, histology, bone segmentation, quantification of bone formation

Publication date: June 30th 2004

Abstract: Micro-computed tomography (µCT) provides quantitative three-dimensional information of bone around titanium implants similar to classical histology. The study, based on an animal model, using cuboid-shaped biofunctionalised Ti6Al4V implants with surrounding bone after 4 weeks, is performed using 3 µCT-systems with X-ray tubes, one synchrotron-radiation-based µCT-system (SRµCT), and classical histology. Although the spatial resolution of the µCTsystems is comparable, only the results of SRµCT agree with results of classical histology. The X-ray tube sources give rise to huge artefacts in the tomograms (interface scattering, beam hardening), which impaired the quantitative analysis of bone up to about 200 µm from the implant surface. Due to the non-destructive character of µCT the specimens can be subsequently examined by classical histology
without restriction. The quantitative comparison of bone formation uncovers the strong dependence of the detected amount of newly formed bone from the selected slice. This
implies the necessity of 3D analysis. SRµCT and classical histology prove that surface modifications of the titanium implant significantly influence the bone formation. Using SRµCT, the preparation artefacts due to cutting and polishing are excluded.

Article download: Pages 42-51. (PDF file)
DOI: 10.22203/eCM.v007a05