2006 Volume No 11– pages 57-75
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Title: Mechanically loaded ex vivo bone culture
system 'Zetos': Systems and culture preparation |
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Authors: CM Davies, DB Jones, MJ Stoddart, K Koller,
E Smith, CW Archer, RG Richards |
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Address: AO Research Institute, AO Foundation, Davos,
Switzerland |
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E-mail: geoff.richards at aofoundation.org |
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Key Words: Bone, culture, histology, immunhistochemistry,
species variation, bioreactor, Technovit |
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Publication date: April 12th 2006 |
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Abstract: This paper introduces the culture preparation
of ovine, bovine and human cancellous bone cores to be used
in an explants model Zetos. The three dimensional (3D) bone
cores were prepared and evaluated for all three animals. Bone
cells in vivo constantly interact with each other, migratory
cells, surrounding extracellular matrix (eCM) and interstitial
fluid in a microenvironment, which continuously responds to
various endogenous and exogenous stimuli. The Zetos system
was designed to culture and mechanically load viable cancellous
bone explants in their near natural microenvironment. This
3D ex vivo system bridges the current gap between in vitro
and in vivo methods. One aim of this work was to compare the
macro and micro-architecture of ovine, bovine and human cancellous
bone tissue in preparation for culture within the Zetos system
in order to determine the optimal source of experimental material.
A second aim was to optimise the preparations of the bone
cores as well as develop techniques involved during tissue
maintenance. Bone core response was visualised using histological
and immunohistochemical methods. The results demonstrate that
cancellous bone explants vary greatly in trabecular density
and bone volume depending on species, age and location. Sheep
and human samples displayed the greatest variation between
bones cores when compared to bovine. Even cores taken from
the same animal possessed very different characteristics.
The histology demonstrated normal bone and cell structure
after the core preparation. Immunohistochemistry results demonstrated
antigen retention after preparation methods.
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Article download: Pages
57-75 (PDF file) |
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