eCM (Eur Cell Mater / e Cells & Materials) Not-for-profit Open Access
Created by Scientists, for Scientists
 ISSN:1473-2262         NLM:100973416 (link)         DOI:10.22203/eCM

2006   Volume No 12 – pages 44-48

Title: Technical Note: Assay of cell quantity in the fibroblast-populated collagen matrix with a tetrazolium reagent

Author: MA Carlson

Address: Department of Surgery, University of Nebraska Medical Center and the Omaha VA Medical Center, Omaha, NE, USA


E-mail: macarlso at unmc.edu

Key Words: fibroblast, collagen, matrix, lattice, wound healing, WST-1, tetrazolium, cellular assay

Publication date: September 28th 2006

Abstract: Determination of cell quantity in three-dimensional culture systems, such as the fibroblast-populated collagen matrix, can be difficult secondary to the abundance of extracellular collagen. The WST-1 assay, which can quantitate cellular processes in monolayer culture, was tested as a measure of relative cell quantity in a three dimensional culture system, the fibroblast-populated collagen matrix. The assay had acceptable accuracy and precision, and was not affected by the extracellular matrix. It was concluded that the assay was a reasonable alternative to other measures of cell quantity in the fibroblast-populated collagen matrix.

 

Article download: Pages 44-48 (PDF file)
DOI: 10.22203/eCM.v012a05