eCM (Eur Cell Mater / e Cells & Materials) Not-for-profit Open Access
Created by Scientists, for Scientists
 ISSN:1473-2262         NLM:100973416 (link)         DOI:10.22203/eCM

2010   Volume No 19 – pages 147-157

Title: Enhancement of silicon using micro-patterned surfaces of thin films

Author: E Kaivosoja, S Myllymaa, V-P Kouri, K Myllymaa, R Lappalainen, YT Konttinen

Address: Department of Medicine, Institute of Clinical Medicine, Helsinki University Central Hospital, Haartmaninkatu 8, FI-00029 HUS, Finland

E-mail: yrjo.konttinen at

Key Words: Biocompatibility, surface modification, micro-patterning, photolithography, osteoblast.

Publication date: April 9th 2010

Abstract: Micro-textured biomaterials might enhance cytocompatibility of silicon-based micro-electro-mechanical system (bio-MEMS) dummies. Photolithography-physical vapour deposition was used to produce diamond-like carbon (DLC) or Ti squares and circles on silicon, and also their inverse replicas; then DLC and Ti were compared for their guiding potential, using a SaOS-2 cell model. Scanning electron microscopy at 48 hours indicated cells were well-spread on large-sized patterns (several cells on one pattern) and assumed the geometrical architecture of underlying features. Medium-sized patterns (slightly smaller than solitary indicator cells) were inhabited by singular cells, which stretched from one island to another, assuming longitudinal or branching morphologies. On small-sized patterns (much smaller than individual cells) cells covered large micro-textured areas, but cellular filopodia bypassed the bare silicon. Immunofluorescence and confocal laser scanning microscopy indicated that the actin cytoskeleton and vinculin-containing adhesion junctions were present on the patterned areas, but not on the bare silicon. Cell density/coverage disclosed a 3.4-3.7-fold preference for the biomaterial patterns over silicon substrate (p < 0.001). Differences in the cellular response between materials were lost at 120 hours when cells were confluent. The working hypothesis was proven; enhancement by micro-patterning depends on the pattern size, shape and material and can be used to improve biocompatibility during the initial integration phase of the device.

Article download: Pages 147-157 (PDF file)
DOI: 10.22203/eCM.v019a15