2010 Volume No 20 – pages 231-244
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Title: Micropatterned hydrogenated amorphous carbon guides mesenchymal stem cells towards neuronal differentiation |
Author: F D’Angelo, I Armentano, S Mattioli, L Crispoltoni, R Tiribuzi, GG Cerulli, CA Palmerini, JM Kenny, S Martino, A Orlacchio |
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Address: Department of Experimental Medicine and Biochemical Sciences, Section of Biochemistry and Molecular Biology, University of Perugia, Italy |
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E-mail: orly at unipg.it or martinos at unipg.it. |
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Key Words: Biological response, cell/protein-material interactions, biomaterial surfaces, tissue engineering, regenerative medicine, cytoskeleton, stem cell differentiation. |
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Publication date: October 5th 2010 |
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Abstract: This study investigated how the design of surface topography may stimulate stem cell differentiation towards a neural lineage. To this end, hydrogenated amorphous carbon (a-C:H) groove topographies with width/spacing ridges ranging from 80/40µm, 40/30µm and 30/20µm and depth of 24 nm were used as a single mechanotransducer stimulus to generate neural cells from human bone marrow mesenchymal stem cells (hBM-MSCs) in vitro. As comparative experiments, soluble brain-derived neurotrophic factor (BDNF) was used as additional biochemical inducer agent. Despite simultaneous presence of a-C:H micropatterned nanoridges and soluble BDNF resulted in the highest percentage of neuronal-like differentiated cells our findings demonstrate that the surface topography with micropatterned nanoridge width/spacing of 40/30µm (single stimulus) induced hBM-MSCs to acquire neuronal characteristics in the absence of differentiating agents. On the other hand, the alternative a-C:H ridge dimensions tested failed to induce stem cell differentiation towards neuronal properties, thereby suggesting the occurrence of a mechanotransducer effect exerted by optimal nano/microstructure dimensions on the hBM-MSCs responses. |
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Article download: Pages
231-244 (PDF file) |
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