eCM (Eur Cell Mater / e Cells & Materials) Not-for-profit Open Access
Created by Scientists, for Scientists
 ISSN:1473-2262         NLM:100973416 (link)         DOI:10.22203/eCM

2011   Volume No 21 – pages 107-121

Title: Cardiogel supports adhesion, proliferation and differentiation of stem cells with increased oxidative stress protection

Author: P Sreejit, RS Verma

Address: Stem Cell & Molecular Biology Laboratory, 201, Bhupat and Jyoti Mehta School of Biosciences, Department of Biotechnology, Indian Institute of Technology Madras, Chennai - 600 036 TN, India

E-mail: vermars at iitm.ac.in

Key Words: Stem cells, proliferation, differentiation, cardiogel, oxidative stress protection, therapeutic biomaterial.

Publication date: January 25th 2011

Abstract: Cultured murine bone marrow derived mesenchymal stem cells (BMSC) when grown along with cardiogel derived from mouse cardiac fibroblast, exhibited increased cell proliferation and differentiation and enhanced survival under oxidative stress induced by the exposure of H2O2 in vitro (similar to in vivo ischemia like condition). Adhesion of BMSC to the cardiogel occurred at a faster rate when compared to the cells grown on normal surface. BMSC attached to cardiogel showed an increased resistance to proteolytic (enzymatic) disassociation. This is the first report on an attempt to use an in house biomaterial for the growth of BMSC that led to their heightened resistance towards oxidative stress. These studies support that cardiogel is an efficient biodegradable three-dimensional extracellular matrix which supports better growth of BMSC and can be used as a scaffold for stem cell delivery, with potential therapeutic applications in cardiac tissue regeneration.

Article download: Pages 107-121 (PDF file)
DOI: 10.22203/eCM.v021a09