2011 Volume No 21 – pages 341-354
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Title: Magnetic resonance imaging tracking of human adipose derived stromal cells within three-dimensional scaffolds for bone tissue engineering |
Author: C Lalande, S Miraux, SM Derkaoui, S Mornet, R Bareille, JC Fricain, JM Franconi, C Le Visage, D Letourneur, J Amédée, AK Bouzier-Sore |
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Address: Inserm U1026, Université Bordeaux Segalen, F-33076 Bordeaux, France |
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E-mail: charlotte.lalande at inserm.fr |
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Key Words: Bone tissue engineering, adipose derived stromal cells, magnetic resonance imaging, human stem cell magnetic labelling, ultrasmall superparamagnetic iron oxide, 3D porous scaffold. |
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Publication date: April 11th 2011 |
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Abstract: For bone tissue engineering, human Adipose Derived Stem Cells (hADSCs) are proposed to be associated with a scaffold for promoting bone regeneration. After implantation, cellularised scaffolds require a non-invasive method for monitoring their fate in vivo. The purpose of this study was to use Magnetic Resonance Imaging (MRI)-based tracking of these cells, labelled with magnetic agents for in vivo longitudinal assessment. hADSCs were isolated from adipose tissue and labelled with USPIO-rhodamine (Ultrasmall SuperParamagnetic Iron Oxide). USPIO internalisation, absence of toxicity towards hADSCs, and osteogenic differentiation of the labelled cells were evaluated in standard culture conditions. Labelled cells were then seeded within a 3D porous polysaccharide-based scaffold and imaged in vitro using fluorescence microscopy and MRI. Cellularised scaffolds were implanted subcutaneously in nude mice and MRI analyses were performed from 1 to 28 d after implantation. In vitro, no effect of USPIO labelling on cell viability and osteogenic differentiation was found. USPIO were efficiently internalised by hADSCs and generated a high T2* contrast. In vivo MRI revealed that hADSCs remain detectable until 28 d after implantation and could migrate from the scaffold and colonise the area around it. These data suggested that this scaffold might behave as a cell carrier capable of both holding a cell fraction and delivering cells to the site of implantation. In addition, the present findings evidenced that MRI is a reliable technique to validate cell-seeding procedures in 3D porous scaffolds, and to assess the fate of hADSCs transplanted in vivo. |
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Article download: Pages
341-354 (PDF file) |
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