2005 Volume No 10– pages 31-39
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Title: Mineralization of regenerated cellulose hydrogels
induced by human bone marrow stromal cells |
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Authors: P. L. Granja, B. De Jéso, R. Bareille,
F. Rouais, C. Baquey, M. A. Barbosa |
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Address: INEB - Instituto de Engenharia Biomédica,
Laboratório de Biomateriais, Rua do Campo Alegre, 823
- 4150-180 Porto, Portugal |
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E-mail: pgranja at ineb.up.pt |
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Key Words: Regenerated cellulose hydrogels, human bone
marrow stromal cells, cell proliferation, mineralization,
calcium phosphates, hydroxyapatite |
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Publication date: October 10th 2005 |
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Abstract: The proliferation of cultured human bone marrow stromal cells (HBMSC) on regenerated cellulose hydrogels was assessed. Regenerated cellulose hydrogels showed good rates of HBMSC proliferation, the cells exhibiting a flattened morphology, and after 22 days in culture, the cells had homogeneously colonized the surface of the materials. Moreover, since the early days in culture, between the surface of the materials and attached cells a continuous granulated hydroxyapatite layer was formed. It has been previously demonstrated in vitro, but without cells, that these materials did not mineralize. Hence, it seems that HBMSC promoted the mineralization of the surface.
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Article download: Pages
31-39. (PDF file) |
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