2007 Volume No 13 pages 11-25
Title: In-vitro interactions of human chondrocytes
and mesenchymal stem cells, and of mouse macrophages with
phospholipid-covered metallic implant materials |
Author: R Willumeit, M Schossig, H Clemens, F Feyerabend |
Address: GKSS Research Center, Max-Planck-Str.1,
21502 Geesthacht, Germany |
E-mail: regine.willumeit at gkss.de |
Key Words: Phospholipid-coatings, porous Ti-6Al-4V, polished
Ti-6Al-7Nb, chondrocytes, mesenchymal stem cells, macrophages,
in vitro tests. |
Publication date: March 2nd 2007 |
Abstract: Phospholipid-coatings on metallic implant
surfaces were evaluated in terms of adhesion, proliferation
and matrix production of skeletal cells, and of macrophage
stimulation. The working hypothesis is that mimicking a model
biomembrane by phospholipids on surfaces to which cells adhere,
the surface recognition by surrounding cells is altered. In
this study, 1) mirror-like polished Ti-6Al-7Nb and 2) porous
Ti-6Al-4V specimens were covered with the phospholipids POPE
(palmitoyl-oleoyl phosphatidyl-ethanolamine) and POPC (palmitoyl-oleoyl
phosphatidyl-choline), and the interactions of a) human articular
chondrocytes (HAC), b) human mesenchymal stem cells (HMSC),
and c) mouse macrophages (RAW 264.7) were tested in vitro.
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Article download: Pages
11-25 (PDF file) |