Title: In vitro and in vivo effects of PDGF-BB delivery strategies on tendon healing: a review

Authors: O Evrova, J Buschmann

Address: University Hospital Zurich, ZKF, Division of Plastic Surgery and Hand Surgery, Sternwartstrasse 14, 8091 Zurich, Switzerland.

E-mail: johanna.buschmann at usz.ch

Key Words: Mesenchymal stem cells, dentine, growth factors, biglycan, decorin, cell expansion, osteoblast differentiation, migration, apoptosis.

Publication date: July 17th 2017

Abstract: To promote and support tendon healing, one viable strategy is the use or administration of growth factors at the wound/rupture site. Platelet derived growth factor-BB (PDGF-BB), together with other growth factors, is secreted by platelets after injury. PDGF-BB promotes mitogenesis and angiogenesis, which could accelerate tendon healing. Therefore, in vitro studies with PDGF-BB have been performed to determine its effect on tenocytes and tenoblasts. Moreover, accurate and sophisticated drug delivery devices, aiming for a sustained release of PDGF-BB, have been developed, either by using heparin-binding and fibrin-based matrices or different electrospinning techniques.
               In this review, the structure and composition, as well as the healing process of tendons, are described. Part A deals with in vitro studies. They focus on the multiple effects evoked by PDGF-BB on the cellular level. Moreover, they address strategies for the sustained delivery of PDGF-BB. Part B focuses on animal models used to test different delivery strategies for PDGF-BB, in the context of tendon reconstruction. These studies showed that dosage and timing of PDGF-BB application are the most important factors for deciding which delivery device should be applied for a specific tendon laceration.

Article download: Pages 15-39 (PDF file)
DOI: 10.22203/eCM.v034a02