eCM (Eur Cell Mater / e Cells & Materials) Not-for-Profit Open Access
Created by Scientists, for Scientists
 ISSN:1473-2262         NLM:100973416 (link)         DOI:10.22203/eCM

2021   Volume No 42 – pages 34-42

Title: Non-contact induction heating and SAAP-148 eliminate persisters within MRSA biofilms mimicking a metal implant infection

Authors: M Verheul, JW Drijfhout, BG Pijls, PH Nibbering

Address: Leiden University Medical Centre, Department of Infectious Diseases C5-P, 2300 RC Leiden, the Netherlands

E-mail: p.h.nibbering at lumc.nl

Abstract: Implant-associated infections are the primary cause of complications following orthopaedic surgery. Due to biofilm and persister formation, current treatments, i.e. surgical debridement followed by antibiotics, often fail. There is an urgent need for alternative strategies to combat such infections. Therefore, the present study investigated the effects of non-contact induction heating (NCIH), the antimicrobial peptide SAAP-148 and combinations thereof on bacterial counts in 7 d mature biofilms and in persister-enriched biofilms of methicillin-resistant Staphylococcus aureus (MRSA) on titanium-aluminium-niobium (TAN) discs. Enrichment of persisters was achieved by daily exposure of mature biofilms to high doses of rifampicin and ciprofloxacin for 3 consecutive days. To heat up the TAN discs, a miniaturised induction heater was built and successfully validated. Using this apparatus, NCIH resulting in surface temperatures up to 85 °C eradicated all the bacteria in immature biofilms but not in mature biofilms, whereas persisters were already eliminated at surface temperatures ≥ 70 °C. SAAP-148 at concentrations > 25.6 µmol/L reduced the persister counts in antibiotics-exposed, mature biofilms. As surface temperatures > 60 °C can have detrimental effects on the surrounding tissues, the maximum temperature of NCIH used in combination with SAAP-148 on persisters was set to 60 °C. Results revealed that this combination was slightly more effective than the peptide or NCIH alone in eliminating biofilm-embedded persisters. NCIH and SAAP-148 can be applied both invasively and non-invasively in various treatment scenarios. Together, combinations of NCIH and SAAP-148 might be a promising treatment strategy to combat metal-implant-associated infections.

Key Words: Implant-associated infection, methicillin-resistant Staphylococcus aureus, biofilm, persisters, titanium-aluminium-niobium discs, non-contact induction heating, antimicrobial peptide, SAAP-148, prosthetic joint infection.

Publication date: July 14th 2021

Article download: Pages 34-42 (PDF file)
DOI:
10.22203/eCM.v042a03

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