2024 Volume No 47 – pages 253-264
Title: Matrix stiffness regulates BMSCs osteogenic differentiation through an autophagy-dependent manner |
Authors: BQ Wang, YQ Yang, HF Xie, CY Xue |
Address: H.F. Xie, Department of Prosthodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, 210029 Nanjing, Jiangsu, China; State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing Medical University, 210029 Nanjing, Jiangsu, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing Medical University, 210029 Nanjing, Jiangsu, China. C.Y. Xue, Department of Oral Implantology, The Affiliated Stomatological Hospital of Nanjing Medical University, 210029 Nanjing, Jiangsu, China; State Key Laboratory Cultivation Base of Research, Prevention and Treatment for Oral Diseases, Nanjing Medical University, 210029 Nanjing, Jiangsu, China; Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing Medical University, 210029 Nanjing, Jiangsu, China |
E-mail: xhf-1980 at 126.com; xuechangyue at njmu.edu.cn |
Abstract: In the field of bone tissue engineering, scaffold materials play a central role in restoring and regenerating the physiological function of the skeletal system. Mechanical signals in the cellular microenvironment significantly influence cellular behavior, and the selection of an appropriate scaffold matrix is crucial in guiding precursor cells towards their desired cell fate. The aim of this study is to investigate the impact of matrix stiffness on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and whether matrix stiffness affects BMSCs osteogenic differentiation in an autophagy-dependent manner. BMSCs were co-cultured with transglutaminase (TG)-Gelatin (Gel) matrices of varying stiffness, and subsequently, osteogenic markers in each experimental group were evaluated. Additionally, the levels of autophagy within the cells were quantitatively measured. Furthermore, inhibitor experiments were conducted to gain deeper insights into potential mechanisms. We observed that within a certain range of stiffness, autophagy levels increased with the augmentation of matrix stiffness, leading to an elevation in the levels of osteogenesis-related molecules. |
Keywords: Bone marrow mesenchymal stem cells, matrix stiffness, extracellular matrix, osteogenic differentiation, autophagy. |
Publication date: June 26th 2024 |
Copyright policy: © 2024 The Author(s). Published by Forum Multimedia Publishing, LLC. This article is distributed in accordance with Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/). |
Article download: Pages 253-264 (PDF file) |
