2024 Volume No 48 – pages 32-44
Title: Brazilin mediates the human periodontal ligament stem cells for periodontitis treatment through the yap signaling pathway |
Authors: JQ Chen, YL Jia, XD Zheng, J Liu, HX Lu, YY Kong, J Zhang, YH Li |
Address: Jun Zhang, Yunnan Key Laboratory of Stomatology, Kunming Medical University, 650504 Kunming, Yunnan, China; Yanhong Li, Department of Preventive Dentistry, Kunming Medical University School and Hospital of Stomatology, 650106 Kunming, Yunnan, China |
E-mail: Zhangjun at kmmu.edu.cn; yanhongli_kmu at 126.com |
Abstract: Objective: To explore the effects of Brazilin on the Human periodontal ligament stem cells (hPDLSCs) mediating periodontal tissue regeneration in periodontitis and the Yes-associated protein (YAP) pathway for periodontitis. Methods: The anti-inflammatory effects and osteogenic effects of Brazilin in the inflammatory microenvironment were mainly evaluated by polymerase chain reaction (PCR), western blot (WB), staining, and the blocking and deletion of the YAP pathway in vitro by verteporfin (VP) and shRNA to evaluated the effect on the YAP pathway. A rat periodontitis model was established and the therapeutic effects were evaluated by Micro-CT, hematoxylin and eosin (HE) staining, Masson staining, and immunohistochemistry. Results: Brazilin effectively down-regulated the expression of inflammatory factors, showed deeper staining, and showed up-regulation of related osteogenic factors, and inhibition of the YAP pathway is followed by inhibition of the osteogenic effects of Brazilin. Brazilin was effective in reducing inflammation and inhibiting alveolar bone loss in the rat periodontitis model. Conclusion: Brazilin can promote periodontal tissue regeneration in periodontitis through the YAP pathway, providing new ideas for the treatment of clinical periodontitis. |
Keywords: Brazilin, human periodontal ligament stem cells, periodontitis, Yes-associated protein. |
Publication date: 8th August 2024 |
Copyright policy: © 2024 The Author(s). Published by Forum Multimedia Publishing, LLC. This article is distributed in accordance with Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/). |
Article download: Pages 32-44 (PDF file) |
